Globally, there are an estimated 3-5 million cholera cases and 100,000-120,000 deaths due to cholera each year. Vibrio cholerae is the etiologic agent of cholera and is mainly spread through contaminated drinking water. It can be fatal within hours, if rehydration is not managed promptly. Inadequate water supplies, poor sanitation and poor hygiene behaviors are largely responsible for cholera diarrhea in resource poor settings. Diarrheal diseases have long been recognized as a major public health challenge in Nepal, putting diarrheal diseases in the second highest research priority area among infectious diseases. Though cholera is considered to be endemic in south Asia, there have been relatively few studies on cholera to date in Nepal. We here report V. cholerae in hospitalized patients with acute diarrhea during July-August, 2012, admitted to Sukraraj Tropical and Infectious Disease Hospital (STIDH), Kathmandu, Nepal. PrimeOA Publications International is one of the leading Open Access Publishers which is running 700+ peer-reviewed journals with quality articles. Journal of Bacteriology & Parasitology is one of the best journals which publish world quality articles. Journal of Bacteriology & Parasitology is a wide range of area that deals with bacterial and parasitic infections.