Tissue Engineering is a scientific field mainly focused on the development of tissue and organ substitutes by controlling biological, biophysical and/or biomechanical parameters in the laboratory. The result corresponds, in most cases, to the elaboration of three-dimensional cellular constructs with properties more similar to natural tissues than classical monolayer cultures. These systems enable the in vitro study of human physiology and physiopathology more accurately, while providing a set of biomedical tools with potential applicability in toxicology, medical devices, tissue replacement, repair and regeneration. To succeed in these purposes, TE uses nature as an inspiration source for the generation of extracellular matrix analogues, either from natural or synthetic origin as well as bioreactors and bio-devices to mimic natural physiological conditions of particular tissues. Tissue engineering is the use of a combination of cells, engineering, and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological tissues.