Next Generation Sequencing & Applications is a peer reviewed medical journal that includes a wide range of fields in its discipline to create a platform for the authors to make their contribution towards the journal and the editorial office promises peer review for the submitted manuscripts to ensure quality. Sequencing is the process of determining the precise order of nucleotides within the genome. It includes various methods or technologies that are used to determine the order of the four nucleotides in the DNA strand. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery