Scholarly journal is a peer-reviewed journal in which scholarship relating to a particular academic discipline is published. Scholarly journals serve as forums for the introduction and presentation for scrutiny of new research, and the critique of existing research. Content typically takes the form of articles presenting original research, review articles, and book reviews. The term Scholarly journal applies to scholarly publications in all fields; this article discusses the aspects common to all academic field journals. Cell culture is the complex process by which the cells are grown under controlled conditions. A consistent presence of floating cells is a common phenomenon in cultures of human embryonic stem cells (hESCs). It is currently believed that unavoidable imperfections in culture conditions lead the cells to undergo senescence and apoptosis resulting in unattached cells floating in the culture medium. Inspired by recent studies on mitotic activities in human embryonic stem cell colonies, we believe the existence of floating cells is not simply the result of unfavorable growth conditions but an intrinsic phenomenon resulted from maintaining the pluripotency of hESCs under the culture conditions. Continuous cell division across the colonies is responsible for the emergence of floating cells during hESC culture. This is quite different from the bacterial colony growth where the cells in the center of colonies are quiescent. Results indicates that continuous cell division, even at the costof floating cells formation, is essential for human embryonic stem cell proliferation.(Chen L, Jin Q, Gong Jand Krishna Dasa SS, The Nature of Floating Cells in Human Embryonic Stem Cell Culture).