Chemistry aids in the development of healthcare, the conservation of natural sources, and the protection of the environment. Chemistry is the important technological know-how, valuable to the knowledge of other sciences and generation. synthetic chemistry, chemical biology, and engineering, leads to tremendous cost and time savings and, therefore, offers a multitude of applications, such as in drug discovery, genomics, proteomics, biomarker discovery, food and environment safety, and point-of-care-medical analysis. Depending on single-use /reuse capability, biochips are made from different rigid substrates such as glass, silicon, quartz, or plastic on which biological material is placed for analysis. At the early beginning, most attention was devoted to DNA chips but later applications involve the use of advanced bioactive surfaces which may control or monitor communication at molecular and cellular level with other biological objects such as proteinspolysaccharides, tissues, living cells, and small organic molecules. The basic principle of operation of a biochip consists of (1) functionalization onto different positions on the chip surface or the channel walls using, e.g., robotic spotting or lithography, (2) biointeraction when the chip is coming into contact with the target compound, (3) readout with an appropriate technique.