Then, in 1828, Friedrich Wöhler published a paper on the synthesis of urea, proving that organic compounds can be created artificially. The dawn of biochemistry may have been in 1833 the discovery of the first enzyme, diastase (also known as amylase), by Anselme Payen. Eduard Buchner contributed to the first demonstration of a complex biochemical process at the outside of a cell in 1896: the alcoholic fermentation based on cells coming from yeast. Although the term biochemistry seems to have been first used in 1882, it is generally accepted that the formal coinage of biochemistry was mentioned in 1903 by Carl Neuberg, a German chemist. Previously, this area would have been referred to as physiological chemistry. Since then, biochemistry has advanced, especially since the mid–20th century, with the development of new techniques such as chromatography, X– ray diffraction, dual polarisation interferometry, NMR spectroscopy, radio isotopic labelling, electron microscopy and molecular dynamics simulations