This is the study of pathways and networks of cellular lipids in biological system it describes the complete lipid profile in cell, tissue, organism. It is a branch of metabolmics. Lipids may be broadly defined as hydrophobic or amphipathic small molecules . Lipidomics produces enormous data and its analysis plays a key role, especially in untargeted studies. As such, robust bioinformatics is critical. Prior to statistical analysis, data preprocessing including signal processing, data normalization and transformation are required, such that raw data are transformed into a format compatible with statistical data analysis