Next-generation sequencing is defined as a process where DNA strands are sequenced parallel and substantially minimize need for the fragment cloning which are used in sanger sequencing of genome. There are different technologies used in next-generation sequencing. The high demand for low-cost sequencing has driven the development of high-throughput sequencing, which is also termed as Next-generation sequencing (NGS). Thousands or millions of sequences concurrently produced in next-generation sequencing process. Next-generation sequencing has become a commodity. Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, American Journal of Respiratory and Critical Care Medicine, Journal of Clinical Endocrinology and Metabolism, Journal of National Cancer Institute, EMBO Journal.