Next-generation sequencing has expanded the frontiers of genomic research, opening up new avenues of enquiry and offering insights into diverse areas of biology. Rapid developments in this field are dramatically lowering costs and increasing speeds. Applications. DNA sequencing may be used to determine the sequence of individual genes, larger genetic regions (i.e. clusters of genes or operons), full chromosomes, or entire genomes of any organism
Next generation methods of DNA sequencing have three general steps:
Library preparation: libraries are created using random fragmentation of DNA, followed by ligation with custom linkers. Amplification: the library is amplified using clonal amplification methods and PCR Sequencing refers to putting events or information in a specific order. The ability to sequence requires higher-order thinking skills, from recognizing patterns to determining cause and effect and more. Sequencing helps students understand and organize material they've learned as well as helps them solve problems