Mycobacterium smegmatis is an acid-speedy bacterial species inside the phylum Actinobacteria and the genus Mycobacterium. it is 3.0 to 5.0 µm lengthy with a bacillus form and can be stained by way of Ziehl-Neelsen method and the auramine-rhodamine fluorescent method. Mycobacterium smegmatis is a Gram-superb bacterium, characterized with the aid of an inner cellular membrane and a thick cellular wall. The Gram-superb microorganism is further labeled as one with a high GC content material and therefore a low AT content material. This fine is used as a crude degree of similarity of different species of microorganism.