Mycobacterium Bovis (M. Bovis) is a slow-growing (16- to 20-hour generation time) aerobic bacterium and the causative agent of tuberculosis in cattle (known as bovine TB). It is related to Mycobacterium tuberculosis, the bacterium which causes tuberculosis in humans. Mycobacterium Bovis is a slow-growing aerobic bacterium and the causative agent of tuberculosis in cattle. It is related to Mycobacterium tuberculosis, the bacterium which causes tuberculosis in humans. M. Bovis can jump the species barrier and cause tuberculosis-like infection in humans and other mammals. Bovis is most commonly found in cattle and other animals such as bison, elk, and deer. In people, M. Bovis causes TB disease that can affect the lungs, lymph nodes, and other parts of the body. bovis could be isolated from 97 rectal pinches followed by 98 pharyngeal swab 2/40 (5%) and 97 fecal samples 1/40 (2.5%) while 1/14 (7.1%) M. tuberculosis isolates were obtained from the pharyngeal swab. Interpretation & conclusion: Among the samples analyzed, PSLG was found to be the most suitable specimen for the isolation of M.