This process is mainly used to determine the mutations in any organisms, if any. The basic procedure is that the primers that are immobilized reach the template and anneal to them by the use of dideoxynucleoside. They are mainly present at a position that is adjacent to the mutant nucleotide. The open access journals are peer reviewed scholarly journals of Next Generation: Sequencing and Application. The top open access journals are freely available on the public internet domain, allowing any end users to read, download, copy, distribute, prink, search or link to the full texts of the articles