A biomarker is a measurable indicator of the severity or stage of the disease. It can also be defined as anything that can be used as an indicator of a particular disease state or physiological state of an organism. Metabolic biomarkers have been introduced recently for the global analysis of all metabolites in a biological sample. The rapidly developing area of metabolomics, which uses analytical techniques such as mass spectrometry and nuclear magnetic resonance. Metabolomics, refer specifically to the analysis of metabolic responses to drugs or diseases