Liquid chromatography used to separate a sample into its individual part, or an analytical chromatographic technique used for the separation of molecules or ions which are dissolved in a mixture or a solvent. High-performance liquid chromatography is a chromatographic technique used to separate the components in a mixture, used mainly to identify each component, and to quantify each component. HPLC is considered as an instrumental technique of analytical chemistry. Separation is based on the analyte’s relative solubility between two liquid phases.