The technique in analytical chemistry used to separate, identify and quantify all the components in the mixture is known as High-performance liquid chromatography. The process utilizes a pump to pass pressurized liquid and sample mixture through a column of sorbent. The different components of the mixture interacts differently with the adsorbent material leading to different flow rate of each component causing their separation, as they flow out of column. The impact factor of journal provides quantitative assessment tool for grading, evaluating, sorting and comparing journals of similar kind. It reflects the average number of citations to recent articles published in science and social science journals in a particular year or period, and is frequently used as a proxy for the relative importance of a journal within its field. It is first devised by Eugene Garfield, the founder of the Institute for Scientific Information. The impact factor of a journal is evaluated by dividing the number of current year citations to the source items published in that journal during the previous two years.