Gene Therapy Research Articles

Gene Therapy Research Articles

The binding of (core) transcription factors critically depends on the recognition of specific DNA sequences, known as DNA motifs. High throughput techniques, such as ChIP-seq and enhanced yeast one-hybrid, which visualizes the interaction of a transcription factor with a bait DNA sequence, are now employed to uncover transcription factor-DNA and DNA-transcription factor interactions, respectively . Such studies show that transcription factor-binding events are abundant and can occur at large genomic distances from genes. Similarly, conserved non-coding sequences can be found throughout the genome. Their conservation implies that they have an important function – they may affect the binding affinity of factors, or encode non-coding RNAs . Interestingly, deletion of these sequences with unknown function can influence gene expression of genes located hundreds of Kb away, implying that long-range looping of DNA brings the sequences into contact with the genes they regulate .


Last Updated on: Nov 26, 2024

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