Incorporation of exogenous DNA into C. Elegans is used for a myriad of purposes. As examples, researchers determine expression of reporter gene constructs, rescue/affirm mutant genes, and analyze gene feature. Germ-line injection is a common approach for transforming C. Elegans. However, the ideal quantity of transgene integrated into transgenic animals can't be controlled via microinjection. To manage for this, researchers alternatively analyze a couple of lines of animals. Another technique for growing transgenics whereby transgenic replica variety is very low is microparticle bombardment (Praitis, 2006). In this technique, transgenic DNA fabric is randomly integrated inside the genome. A 0.33 technique for creating transgenic C. Elegans whereby transgenes are inserted at centered genomic sites, in single replica, is through mobilization of a Mos1 insertion (Frøkjær-Jensen et al., 2008).