Bioprospecting is that the exploration of biodiversity for brand spanking new resources of social and commercial value. Ecologists are involved in three vital ways: first, applying ecological principles to the invention of latest resources. during this context, explanation becomes a huge economic database.Bioprospecting of orthologous genes from a wide array of plant species offers a valuable alternative to protein engineering and directed evolution. While enzyme variants corresponding to any enzymatic step within a target pathway can be screened to enhance catalytic efficiency, pathway bottlenecks present the most promising opportunities for enzyme bioprospecting. BIA biosynthetic gene variants can be easily identified by querying genome and transcriptome databases of BIA-producing plants, including the 1000 Plants Project and the PhytoMetaSyn Project described briefly in Section 2. Due to the mounting availability of DNA sequence and transcriptome data available within the public domain, enzyme bioprospecting offers vast opportunities for enhancing overall BIA pathway efficiency by tapping into nature's metabolic diversity.