Dye agar plates were readied utilizing PDA containing 60mg L-1 of every individual color. Plates were immunized with a 1 cm 2 bit of the parasite segregated from a multi day PDA plate development, cut out from the effectively developing contagious culture. Color agar plates that were not immunized filled in as controls. All plates were hatched at 28¡ÆC. Following six days, the size of the decolorization corona was estimated. Color agar plates were readied utilizing PDA containing 60mg L-1 of every individual color. Plates were vaccinated with a 1 cm 2 bit of the parasite separated from a multi day PDA plate development, cut out from the effectively developing contagious culture. Color agar plates that were not vaccinated filled in as controls. All plates were brooded at 28¡ÆC. Following six days, the size of the decolorization corona was estimated. Assessment of the proteinaceous antigen-counter acting agent response by precipitin development in agar plates is one of the most widely recognized apparatuses in immunology. It is regularly fundamental to track the response or number of precipitin lines in a plate for similar purposes. The most widely recognized techniques for protection are by portraying or capturing the precipitin lines. Both of these techniques have inconveniences. The previous strategy is dreary and requires significant practice to accomplish precise records. The subsequent strategy is fantastic for recording thick precipitin lines yet requires an impressive photographic method for recording faint lines.