Biological stains from two or more individuals will result in a mixed genotype if they cannot be separated prior to DNA analysis. Mixture interpretation is often very complex. Moreover, mixed genotypes have a lower probative value and are difficult to convince a jury. Therefore, in biological evidence containing cells from different individuals, successful separation of the offender’s cells from those of the victim is very helpful for unambiguous genotyping. Even in a two-person mixture, when there are shared alleles between the major and the minor profiles, the interpretation becomes difficult—especially in mixtures where the minor profile is less than one third of the level of the major profile. Therefore, the development of separation methods reduces the need for mixture interpretation. Forensic scientists can use DNA profiles to identify criminals or determine parentage. A DNA profile is like a genetic fingerprint. Every person has a unique DNA profile, making it very useful for identifying people involved in a crime. The only exception to this is identical twins. The laser capture microdissection (LCM) technology represents a significant improvement in cell separation methods. It combines existing light microscopic instrumentation with laser beam technology and allows targeting of specific cells or tissue regions that need to be separated from others. Isolation of these cells or tissue sections occurs, under direct visualization, into separate tubes for direct DNA extraction and analysis.