Column chromatography in chemistry is a chromatography approach used to isolate a single chemical compound from a mixture. Chromatography is adequate to separate substances established on differential adsorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be free into fractions. The technique is widely applicable, as many different adsorbents can be used with a wide range of solvents. The approach can be used on scales from micrograms up to kilograms. The main asset of column chromatography is the almost low cost and disposability of the stationary phase used in the process. The latter avert cross-contamination and stationary phase degeneration due to convert. Column chromatography can be done using gravity to action the solvent, or using compressed gas to push the solvent through the column