Toxoplasma gondii (T. gondii), is an obligate intracellular single-cell parasite that survives only if infects worm blood animals. It is a global health condition that almost infects almost one-third of total world population . In Sudan, scarce data available about T. gondii in total population, however, 34% of the pregnant women were seropositive for Immunoglobin G anti-toxoplasma antibodies . In general, the major route of infection is the oral route by ingestion of sporulated oocysts in contaminated food or drinks . Trans- placental transmission from the pregnant women to the fetus is another possible route of infection . In women who are infected with T. gondii during pregnancy and not treated, the incidence of fetal infection is 25% in the first trimester, 54% in the second trimester and 65% in the third trimester . Pregnant women infected with T. gondii have variable disease outcomes that include miscarriage or stillbirth. Vertical transmission can cause congenital toxoplasmosis, which is characterized by chorioretinitis, hydrocephalus and cerebral calcification . On the other hand, infected pregnant women with T. gondii usually asymptomatic and therefore diagnosis can be delayed and adverse outcome may occur. Acute and latent T. gondii infections during pregnancy are most commonly diagnosed by detecting the immunoglobulin IgG and IgM in the serum samples of the patients. Thus, early screening and laboratory diagnosis of toxoplasmosis of paramount importance in order to prevent the congenital toxoplasmosis.