The separation of one or more cell types from other cells is a critical step in biological research and clinical testing. By conducting experiments on an isolated population of cells, scientists can perform a variety of downstream applications, answer research questions, and conduct experiments without interference from other cell groups. Positive selection, Depletion and Negative selection are the Three Cell Separation Methods. Density gradient centrifugation, Filtration, FACS (fluorescence-activated cell sorting), and MACS (magnetic-activated cell sorting) are the Common Cell Isolation Technologies