Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. Cell cultures obtained from immortal cell. These cell line carry out for the carcinogenic cell. These cultures generally used for the laboratory purpose to avoid cell harvesting from the host cell. This kind of cell can be culture and store for the future generations. A cell can undergo 50 divisions and it become inactive, so cell lines are useful for research purpose. High-impact journals are those considered to be highly influential in their respective fields. The impact factor of journal provides quantitative assessment tool for grading, evaluating, sorting and comparing journals of similar kind. It reflects the average number of citations to recent articles published in science and social science journals in a particular year or period, and is frequently used as a proxy for the relative importance of a journal within its field. It is first devised by Eugene Garfield, the founder of the Institute for Scientific Information. The impact factor of a journal is evaluated by dividing the number of current year citations to the source items published in that journal during the previous two years.
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