Monitoring and controlling of the process parameters is very essential in all industries and effective methods to monitor those parameters are need to be developed, optimized and need to be maintained at their maximum efficiency in order to meet the desired product yield and product quality. Varieties of products are being manufactured through biotechnological processes which include various primary and secondary metabolites, tissues, vaccines, therapeutic proteins etc. Various factors are involved in cultivation of cells and production of the desired product by biotechnological process which includes, medium composition, bioreactor designing, identifying and controlling very minute parameters in narrow ranges in order to obtain the desired product quality and productivity.