Microbial biofilms have generally been examined as a stimulus for the settlement of macrofouling organisms Microbial biofilms are communities of microorganisms attached to a living or inert surface in an aqueous environment and surrounded in a matrix of extracellular polymeric substances . The EPS synthesized by microbial cells consist of polysaccharides, proteins, glycoproteins, glycolipids and, in some cases, extracellular DNA. These substances form a network that influence the porosity, density, water content, charge, sorption, hydrophobicity, and mechanical stability of biofilms. In addition, the extracellular matrix provides nutrients for biofilm cells through interaction with the environment. Oral biofilms are polymicrobial and formed by more than 700 different species of microorganisms, and their composition may vary according to different substrates (soft shedding tissues and hard nonshedding surfaces) within the oral cavity