Fungi will manufacture many living thing enzymes thereby facilitating the degradation of recalcitrant compounds in vinasse. thin fungi need straightforward method for plant biomass separation consequently plant biomass cost can be comparatively low. In general, as a result of plant biomass has comparatively high supermolecule content around five hundredth (on dry wt. basis), the most purpose of plant method is for manufacturing single cell supermolecule (SCP) for food/feed applications. Food-grade thin fungus: R. oligosporus is understood as a beginning culture for creating “tempeh†associate Indonesian delicacy.