The Journal of Microbial and Biochemical Technology is an academic journal that provides an opportunity to share information about new technologies and their applications among the medical scientists and researchers. Microbes are recognized for their diverse metabolic activity and ubiquitous presence, unique strategies of survival under extreme conditions, and important impacts on human health and technology. Biochemical technology enables us to understand the enzymatic, protein, bioinformatic, and biochemical mechanisms of microbes. The Journal of Microbial & Biochemical Technology is a scientific journal that addresses current research related to various aspects of microbial biochemical processes. The journal is published as part of the PrimeOA Publications publishing group, which provides a gateway to free journals that enable free online access to full text and highly graphic articles published in current and preceding years volumes. The journal includes a wide range of fields in its discipline, to create a platform for authors to make known their contributions towards the advancement of microbial and biochemical technology and scientific knowledge. As a scholarly Open Access journal, the Journal of Microbial & Biochemical Technology aims to publish rapid, complete and reliable information on technology and current developments in the mode of original articles, review papers, case reports, short communications, etc. The journal and the editorial office promise an efficient and careful peer review process for all submitted manuscripts, to assure high quality of all published papers, following which all published papers become freely available online without any restrictions or any other subscriptions to researchers worldwide. By this means, the Journal of Microbial & Biochemical Technology aims to be one of the best and most highly respected Open Access journal of scholarly publishing. The journal is using the Editorial Managing System to assure a high quality and efficient peer review process. Editorial Tracking is an online manuscript submission, review and tracking system. Review processing is performed by the editorial board members of Journal of Microbial & Biochemical Technology or outside experts; at least two independent reviewers approval followed by editor approval is required for acceptance of any citable manuscript. Authors may submit manuscripts and track their progress through the system, hopefully to publication. Reviewers can download manuscripts and submit their opinions to the editor. Editors can manage the whole submission/review/revise/publish process.Within the past few months we have lost, through death, two of the original members of the Editorial Board of Biochemical Medicine-Dr. Carl Cori and Dr. Sidney Colowick. We have known both of these scientists for more than forty years and they are inextricably linked in our memory. We first met in Dr. Cori’s laboratory at Washington University in 1942. Dr. Sidney Colowick was a postdoctoral student. The tone of this remarkable laboratory was set by the Coris who had gathered around themselves a group of people which included Gerhardt Schmidt, Luis Leloir. Earl Sutherland, Ed Krebs, Milton Slein, Lou Berger, Ed Hunter, and Arta Alden Green. The excitement of working in the presence of this stimulating group of people was overwhelming. Many interesting discussions took place around the battered old library table in the diminutive conference room. Meyerhoff and Tiselius visited and lectured informally on their developing work. Everyone who was anybody in biochemistry came by. This was a period when the broad outlines of physiological chemistry were being forged and the few English-language journals such as Biochemical Journal, Nature, and Journal of Biological Chemistry were filled with the work of Krebs and Quastel and Houssay, with whom Cori shared the Nobel Prize in 1946. Sidney went to Johns Hopkins University where he became a member of the McCollum Pratt Institute which developed into a major center of biochemical research in the United States. An annual highlight of this group was the McCollum Pratt Symposium, to which all of us looked forward each year. Sidney teamed up with Nate Kaplan in the McCollum Pratt Institute to produce many outstanding discoveries and the most important handbook of biochemistry ever written, “Methods in En- zymology” by Colowick and Kaplan. The relation between Cori and Colowick was as father and son in science. The first time we ever noted it was in 1942 when Sidney was walking down the dark corridor in the Department of Chemistry, Washington University, carrying a brand new 300-millimeter glass desiccator with tubulated top and glass stopcock, perhaps one of the most expensive items in the laboratory. Dr. Cori popped out of his office and Sidney stopped short. The top of the desiccator slid off and crashed to the floor at his feet. Sidney made remarks about paying for the dessicator and Dr. Cori said, “It’s alright, Sidney, we all break things aroA single intraperitoneal injection of 5 mg (250 mg/kg body wt) streptozotocin induced overt diabetes within 48 hr in male, 6-week-old mice of C57BL6 and C57BL10 (sensitive) strains. The C3HHeJ and C3Heb (resistant) strains exhibited a progressive delayed-onset hyperglycemia which required 6 weeks to reach the level seen within only 48 hr in sensitive strains. Resistance to SZ-induced diabetes was not related to changes in body weights or different pharmacokinetics of the drug. C3HHeJ (resistant) mice showed a smaller fall in immunoreactive insulin pancreatic content and less severe damage of pancreatic islets on histologic examination, when compared to C57BL6 (sensitive) mice during the first week post-SZ. These results suggest that strain-related resistance to SZ-induced diabetes may be mediated at the level of the β cell's responsiveness to the cytotoxic action of the drug. This very simple experimental tool may be of value in monitoring the period before the onset of overt insulin-deficient diabetes and for probing the nature of factors that constitute a genetically resistant pancreas, understanding of which could aid management of diabetes mellitus in humans.
Carbamazepine, a drug which is widely used in neurological diseases, has a porphyrogenic effect in chick embryo liver cells in culture. It increased the concentration of cellular porphyrins by 80-fold and δ-aminolevulinate synthase activity by 4-fold. The increase in the accumulation of porphyrins preceded that of ALAS activity. Measurements of the activities of aminolevulinate dehydrase, porphobilinogen deaminase, and uroporphyrinogen decarboxylase showed that C inhibits UROD up to nearly 50% and PBGD activity up to 20%, but does not affect the activity of ALAD.
The pattern of accumulation of porphyrins, mainly uro- and heptacarboxylporphyrin, is compatible with an inhibition of UROD. We may, therefore, conclude that the porphyrogenic effect of C in monolayers of chick embryo liver cells is the sesult of its inhibitory effect on the activity of UROD.he pattern of acid proteinase zymogens, seven pepsinogens (Pg) and slow moving protease (SMP), in normal human gastric mucosa has been reported. No significant differences were found in appearance of individual pepsinogens in oxyntic mucosa in the two sexes, but in pyloric mucosa, Pg 3 occurred significantly more often in men. Rapidly migrating pepsinogens (constituents of Group I pepsinogens) were seen in pyloric mucosa as well as in oxyntic mucosa. The duodenal mucosa contained small amounts of proteinases, the activity being largely confined to the slower moving proteinases (constituents of Group II pepsinogens).