batch culture A technique used to grow microorganisms or cells. A limited supply of nutrients for growth is provided; when these are used up, or some other factor becomes limiting, the culture declines. Cells, or products that the organisms have made, can then be harvested from the culture.
Batch culture, In the broadest sense, outlined as associate operational technique in biotechnological processes wherever one or a lot of nutrients (substrates) area unit fed (supplied) to the bioreactor throughout cultivation and within which the product(s) stay within the bioreactor till the tip of the run. An alternate description of the strategy is that of a culture within which "a base medium supports initial cell culture and a feed medium is intercalary to forestall nutrient depletion".It is additionally a sort of semi-batch culture. In some cases, all the nutrients area unit fed into the bioreactor. The advantage of the fed-batch culture is that one will management concentration of fed-substrate within the culture liquid at haphazardly desired levels (in several cases, at low levels).
A large-scale closed system culture in which cells are grown in a fixed volume of nutrient culture medium under specific environmental conditions (e.g. nutrient type, temperature, pressure, aeration, etc.) up to a certain density in a tank or airlift fermentor, harvested and processed as a batch, especially before all nutrients are used up.
Batch culture systems provide a number of advantages:
1. Reduced risk of contamination or cell mutation as the growth period is short.
2.Lower capital investment when compared to continuous processes for the same bioreactor volume.
3.More flexibility with varying product/biological systems.