Serum bactericidal assays measure the practical activity of antibodies and were used for many decades. SBAs at once degree antibody killing interest with the aid of assessing the ability of antibodies in serum to bind to bacteria and spark off supplement. This supplement activation outcomes inside the lysis and killing of the target bacteria. These assays are treasured because they go beyond quantifying antibody production to clarify the organic capabilities that these antibodies have, allowing researchers to take a look at the role that antibodies may additionally play in preventing. SBAs had been used to study immune responses for lots human pathogens, but there's no broadly frequent methodology for Shigella at gift. Historically, SBAs have been very exertions-intensive, requiring many time-eating steps to as it should be quantify surviving bacteria. This protocol describes a easy, sturdy, and excessive-throughput assay that measures purposeful antibodies unique for Shigella in serum in vitro. The technique described right here offers many blessings over traditional SBAs, such as the usage of frozen bacterial shares, ninety six properly assay plates, a micro-subculture system, and automated colony-counting. All of those adjustments make this assay much less hard work-extensive and more high-throughput. This protocol is easier and faster to perform than conventional SBAs at the same time as nonetheless using easy technology and comfortably to be had reagents. The protocol has been successfully carried out in more than one independent laboratories and the assay is robust and reproducible. The assay can be used to evaluate immune responses in pre-clinical as well as medical studies. Quantifying shigellacidal antibody titers both earlier than and after antigen exposure (both by using immunization or contamination) permits for a broader understanding of ways practical antibody responses are generated and their contribution to protective immunity. The improvement of this standardized, properly-characterized assay may additionally substantially facilitate Shigella vaccine layout. bactericidal assay has been successfully publishing quality Research articles from many years and looking forward to frame up eminent, outstanding issues with best quality research articles in this year. We request you to kindly submit and publish your paper in this best journal and get global acknowledgment