Biological specimen was the comprehensive analysis of metabolites which is derived from Metabolomics. A small number of metabolites used to diagnose diseases like monogenic disorders such as inborn errors of metabolism.Metabolomics is an emerging field and is broadly defined as the comprehensive measurement of all metabolites and low-molecular-weight molecules in a biological specimenThe most common techniques used in metabolomics for data acquisition are nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS), often applied in combination to extract from samples as much information as possible,The Human Metabolome Database is a freely available, open-access database containing detailed data on more than 40,000 metabolites that have already been identified or are likely to be found in the human body.Essential metabolites are identified under nineteen environments specified by combinations of different carbon, phosphate, nitrogen, and sulfate sources, and aerobic/anaerobic conditions (SI Table 2). ... 1-A) Metabolites which are identified as essential for all nineteen growth conditions.