The study was to understand Angiostrongylus cantonensis infection status in host animals and populations in Shenzhen. In 2016–2017, 10 different ecological environments were selected, and intermediate and definitive hosts collected at the sites were examined using the enzyme digestion and dissection method to determine their infection status. Meanwhile, serum was collected from outpatients and healthy people. The enzyme-linked immunosorbent assay test was performed to detect serum IgG-specific antibodies to A. cantonensis, and serological characteristics of the populations were analyzed. A total of 300 Achatina fulica samples had an A. cantonensis infection rate of 10.67% (32/300) and an average infection intensity of 68.7 per snail, whereas 302 Pomacea canaliculata samples had an infection rate of 6.29% (19/302) and an average infection intensity of 31.4 per snail.