An anaerobic organism or anaerobe is any organism that doesn't require oxygen for growth. it's going to react negatively or maybe die if free oxygen is present. In contrast, an aerobic organism is an organism that needs an oxygenated environment. Anaerobes could also be unicellular or multicellular. Since normal microbial culturing occurs in atmospheric air, which is an aerobic environment, the culturing of anaerobes poses a drag . Therefore, variety of techniques are employed by microbiologists when culturing anaerobic organisms, for instance , handling the bacteria during a glovebox crammed with nitrogen or the utilization of other specially sealed containers, or techniques like injection of the bacteria into a dicot plant, which is an environment with limited oxygen. The GasPak System is an isolated container that achieves an anaerobic environment by the reaction of water with sodium borohydride and bicarbonate of soda tablets to supply hydrogen gas and CO2 . Hydrogen then reacts with oxygen gas on a palladium catalyst to supply more water, thereby removing oxygen gas. the difficulty with the Gaspak method is that an adverse reaction can happen where the bacteria may die, which is why a thioglycollate medium should be used. The thioglycollate supplies a medium mimicking that of a dicot, thus providing not only an anaerobic environment but all the nutrients needed for the bacteria to thrive.