Affinity chromatography is a method of separating biochemical mixture based on a highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid. It is a type of chromatographic laboratory technique used for purifying biological molecules within a mixture by exploiting molecular properties, e.g. protein can be eluted by ligand solution. Biological macromolecules, such as enzymes and other proteins, interact with other molecules with high specificity through several different types of bonds and interactionChromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis.
It is a separation technique in which a mobile phase carrying a mixture is caused to move in contact with a selectively absorbent stationary phase. Affinity chromatography is a type of liquid chromatography for the separation, purification or specific analysis of sample components. It utilizes the reversible biological interaction or molecular recognition called affinity which refers to the attracting forced exerted in different degrees between atoms which cause them to remain in combination.