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Experts in Genetics
Sanjay Gupta Aimée M. Dudley David Buchanan Drena Dobbs Karin S Dorman Dr. Xun Gu Thomas Peterson IRFAN AHMAD ANSARI John Golin Jannice Friedman Vimla Aggarwal Andrea Rinaldi Carmen Argmann Eleanor Maine Albertini Alessandra Judith Greenberg Mark Clark John F. Hayes Ashok Kumar Roger I. Cue View MoreInvestigation of the molecular mechanisms leading to genome-wide DNA demethylation. Role of Tet3 protein and 5-hydroxymethylcytosine (5hmC) during epigenetic reprogramming in mouse zygotes. Technical expertise: - Designed and validated cellular assays to estimate the potency and the specificity of small molecule inhibitors (cofactor or nucleoside analogues) towards their specific targets. - Developed high level of expertise in handling complex cellular culture systems such as embryonic stem cells and in vitro fertilisation-derived embryos. - Designed CRISPR/Cas targeting vectors for the construction of knockout murine models. Method development, implementation and validation: - Developed and optimised a novel and highly sensitive LC/MS method to quantify DNA modifications that was adapted for minute samples. - Optimised fluorescence-based methods for the visualisation of nucleoside and metabolite analogues by confocal microscopy.
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