Principal Investigator
Molecular Neurobiology
The Instituto de Neurociencias de Alicante
Spain
Degree: Graduated in Chemistry for the University of Alicante in 1985 Ph.D program: 1989, he obtained his Ph D degree in the Neuroscience program of the Instituto de Neurociencias Posdoctoral Positions: From 1990 to 1992 he conducted post-doctoral studies in Dr. Marlene Hosey´s lab at Northwestern University in Chicago. At the end of 1992 he returned to Alicante after receiving a Re-incorporation fellowship. He started then his independent research lines and joined the Instituto de Neurociencias as investigator. Previous positions: Current Position: He currently participates in several research projects in collaboration with other members of the Molecular Neurobiology Unit of the Institute and is a full professor in the area of ​​Biochemistry and Molecular Biology of the Miguel Hernandez University.
Research Fields: Molecular mechanisms of exocytosis in a neuroendocrine model: the involvement of cytoskeleton and SNARE complex proteins Adrenomedullary chromaffin cells have been used as an excellent experimental model to study the exocytosis and therefore the molecular mechanisms of neurotransmission. It is now clear that the proteins involved in the processes of vesicle docking, membrane fusion and neurotransmitter release are common to many cellular systems (SNARE hypothesis). Our research interest is focused in two different aspects of the molecular mechanisms of neurotransmission: Implication of molecular motors such myosin-actin in vesicle transport during neurosecretion and the determination of essential aminoacids of synaptobrevin or SNAP-25 implicated in the process of membrane fusion. Experimental approaches involve strategies using antibodies, sequence peptide design and protein overexpression that demonstrate the participation of specific protein domains in exocytosis. In addition, the role of these proteins on the secretory stages have been studied using amperometry, technique that resolves single fusion events