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Toshiyuki Usami

Associate Professor
Bioresource Science
Chiba University
Japan

Professor Agri and Aquaculture
Biography

Dr. Toshiyuki Usami is a phytopathologist. One of his research themes is “Genetic studies on pathotypes of Verticillium dahliae”. V. dahliae causes wilt disease on various plants. Although the host range of this phytopathogenic fungus is wide, the pathogenicity differs among isolates. Japanese isolates of V. dahliae is divided into several pathotypes on the basis of their pathogenicity. For example, tomato pathotype is pathogenic on tomato; but is nonpathogenic on sweet pepper. Dr. Usami is investigating the pathogenicity-differentiating mechanisms of V. dahliae by comparing genetic profiles among pathotypes. He obtained several “tomato pathotype-specific” DNA sequences, and revealed that these sequences are located on a same chromosome.

Research Intrest

Studies on expression of disease-resistance genes in plant, analysis of microbial community in soil using DNA sequences and characterization of new variety of fungal pathogen.

List of Publications
Morita, K., Kimura, S., Saito, M., Shinoyama, H., Usami, T., Amemiya, Y., Shishido, M. (2005): Generation and characterization of reduced virulence Fusarium oxysporum f. sp. lycopersici mutants through plasmid-vector insertion. Mycopathologia, 160, 67-73
Shishido, M., Yoshida, N., Usami, T., Shinozaki, T., Kobayashi, M., and Takeuchi, T. (2006): Black root rot of cucurbits caused by Phomopsis sclerotioides in Japan and the grouping of the pathogen based on the phylogenetic analysis. Journal of General Plant Pathology, 72: 220-227
Usami, T., Ishigaki, S., Takashina, H., Matsubara, Y., Amemiya, Y. (2007): Cloning of DNA sequences specific to the pathotype and race of Verticillium dahliae. Journal of General Plant Pathology, 73: 89-95
Usami, T., Fukaya, M., Amemiya, Y. (2007): Electrophoretic karyotyping and mapping of pathotype-specific DNA sequences in Japanese isolates of Verticillium dahliae. Journal of General Plant Pathology, 73(6)

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