He is currently working as a Professor in National chiayi university in Department of Applied Chemistry. He has completed his Ph.D. from University of Cape Town. His research Area is Biochemistry. His research interest has been focused on elucidating the mechanism of action and the structure-function relationship of enzymes. One ongoing project is to develop an effective enzymatic method for synthesizing theanine from glutamine and ethylamine by Escherichia coli g g -glutamyltranspeptidase ( g -GGT). Moreover, the conserved residues of recombinant E. coli g -GGT have been replaced with other amino acids by site-directed mutagenesis. The preliminary results show that some of the residues are involved in the autocatalytical processing of the enzyme. We also cloned and expressed other g -GGTs from Bacillus stearothermophilus and B. kaustophilus in recombinant E. coli . The overproduced enzymes have great potential for the bioconversion of medical agents. The specific aims include identifying phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinase from Bacillus sp. TS-23, analyzing the functions of the conserved amino acids of microbial leucine aminopeptidases (LAPs), and recovering the industrial important enzymes by adsorption-elution on raw starch. The other ongoing project is to study the structure-function relationship of B. stearothermophilus LAP. To study the catalytic mechanism of this enzyme, B. stearothermophilus LAP is subjected to structural analysis by X-ray crystallography and to functional analysis by mutagenesis. By combing the data from both approaches, we hope that the molecular action of LAP can be appreciated in the near future.　

mechanism of action and the structure-function relationship of enzymes