Washington University Biochemistry and Enzymology, Synthesis of Nucleic Acid Derivatives, Proteomics We are currently studying the Nudix hydrolase family of enzymes. This is a large, widely distributed class of proteins that we have discovered having the common signature sequence: GX6EX7REUXEEXU (U= Ile, Leu, or Val) At present, over 600 members of the family have been identified in more than 200 species by BLAST searches of the data banks, and this number is increasing daily. We are systematically cloning selected members of the family, expressing them in E.coli, purifying the proteins and attempting to identify their function. This seemingly difficult task has been made practicable by our discovery that the common feature of the substrates of these enzymes is that they are all Nucleoside diphosphates linked to some other moiety, x, hence the acronym, Nudix. These enzymes can be thought of as surveillance proteins, because their substrates are either potentially toxic to the cell, are signaling molecules, or intermediates whose concentrations require modulation during cellular metabolism.

Washington University Biochemistry and Enzymology, Synthesis of Nucleic Acid Derivatives, Proteomics We are currently studying the Nudix hydrolase family of enzymes. This is a large, widely distributed class of proteins that we have discovered having the common signature sequence: GX6EX7REUXEEXU (U= Ile, Leu, or Val) At present, over 600 members of the family have been identified in more than 200 species by BLAST searches of the data banks, and this number is increasing daily. We are systematically cloning selected members of the family, expressing them in E.coli, purifying the proteins and attempting to identify their function. This seemingly difficult task has been made practicable by our discovery that the common feature of the substrates of these enzymes is that they are all Nucleoside diphosphates linked to some other moiety, x, hence the acronym, Nudix. These enzymes can be thought of as surveillance proteins, because their substrates are either potentially toxic to the cell, are signaling molecules, or intermediates whose concentrations require modulation during cellular metabolism.