A.I. Yaropolov

Head of Laboratory
Laboratory of Chemical Enzymology
AN Bach Institute of Biochemistry
Russian Federation

Professor Biochemistry
Biography

He has great experience in bioelectrocatalysis, in studies of the mechanism of redox enzymes and free radical reactions, in development of heterogeneous catalysts based on immobilized enzymes, creation of various types of biosensors. He is one of the authors of the discovery called “bioelectrocatalysis” of oxidoreductases (laccase, peroxidase, hydrogenase). He is the author of 8 patents and more that 130 publications in refereed journals

Research Intrest

- physico-chemical study of oxidoreductases and their application in biotechnology; - investigation of the mechanism of free radical reactions during oxidoreductase function; - delicate organic synthesis using oxidoreductases; - bioelectrochemical studies of oxidoreductases, including direct (mediator-less) electron transfer and bioelectrocatalysis; - biosensors and biofuel cells; - biodegradation of xenobiotics: selection and investigation of redox mediators for the degradation of xenobiotics using laccase-mediator systems. Main directions of the present activity: - study of homogeneous and heterogeneous catalysis of multicopper oxidases including bioelectrocatalysis; possible application of fundamental knowledge for the creation of biosensors and biofuel cells. - green synthesis of electroconductive polyaniline by oxidoreductases Basic accomplished projects: 1. Studies of electrochemical properties of monoamino oxidase in reactions with artificial acceptors of electrons. 2. Investigation of free radical reactions in laccase-mediator systems under enzymatic degradation of xenobiotics and pulp biobleaching. 3. Development and study of mechanism of environmentally friendly synthesis of water-soluble electroconductive polyaniline by laccase and peroxidase. Current projects: 1. Possible application of laccases in green organic synthesis 2. Novel biosensors and analysis kits based on genetically engineered biomolecules for formaldehyde assay

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